Topics for: Characterization of the Tomato (Solanum lycopersicum) Pectin Methylesterases: Evolution, Activity of Isoforms and Expression During Fruit Ripening

Focus on the practical applications of understanding PME activity. How can scientists and plant breeders use this genetic knowledge (e.g., by modifying or silencing specific PME isoforms) to develop new tomato varieties with better texture, longer shelf life, or improved processing quality?

Explore the concept of PME Isoforms (like PE1 and PE2) and Evolution. Why does the tomato plant have many different PME genes, and how do these different versions (isoforms) perform different functions in the fruit versus the leaves or roots?

Discuss the direct connection between Pectin Methylesterases (PMEs) and the softening of a tomato fruit. How does this enzyme change the cell wall structure (specifically pectin) and what does the timing of its activation tell us about controlling a tomato's shelf life?